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Previous Versions

Previous Versions

Space Ranger 4.0.1 (Jun 12, 2025)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 885 MB
    md5sum: b0beb100947717cee8c7ad0d7a9f0247
    curl -o spaceranger-4.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-4.0.1.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Tt07OYBA97CMaFQvtPtx9VRHmsnCzObqbbgHhnc9myAF2YAI6mkKraOHZHcfWEBab-~ceG~mDZTe-MyMfT1fTEjrg6NYGq7GlimE~rbgl92W62k260vIP6WnhjQhOtdLlIDjvN5gs7w2Ij0omgLDoh7fVwrIZSKh8jq5edAyQ5PZHnh1NAVsgrdrJXhpSaU826DHSDjopYPO0p9mnU-~DB0BmkM3DwaefyzYX~qHTSpb0vdRMiUjUNZDhCxxKJnT5n6iKSTc6JCCqUjTb9bJRwEhADJGjmvUKfsYzAmwSNBW9knmwFBFGxTCZF8eFRTgUtzmYTpk7u5zsMAtlKrW~A__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 582 MB
    md5sum: 3610ff948a6f8129cf49509f1bb34970
    curl -o spaceranger-4.0.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-4.0.1.tar.xz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=YB6oOC8Zt7tQEO3qTxgPzBcwQPx0lvFbRBYyFLsHDafNlkDzybLiJ~wSAwRrh4z9kVZQPY2z65cBX20kjpRLpndSw7j6vh3Iz8mczBJ~Da3kGi5kYmIJvPh-VTD5HaxbZgWQaRjMuQ8kh1v0J1vvmsLALGI6L~cAG8JVjRgifNQs2hA5MhDlzFjS3LrqzEMtL6gxrtEZMSPfHCJbxjt2vYdpnZ8Ov5WLPuRXWhpkP01PPmgS5heLo9nZmj-9~rWWzF1jSUTcZx~Y34hZnfI15EhSCezj6T7eGgLRd9zRFx8ThCODwvJqAaOBtEWSHYvOmg66L260~4CSZKComa8Mzw__"

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Space Ranger v4.0.1 highlights

  • Space Ranger v4.0 now supports Visium HD 3' Gene Expression!
  • Cell and nucleus segmentation are now included for Visium HD and Visium HD 3' data with H&E images.
  • Space Ranger v4.0 now features a new UMI-based registration algorithm for Visium HD and Visium HD 3' data.
  • Starting with v4.0, anonymized telemetry data is collected and sent to 10x Genomics.
Release notes

Space Ranger 3.1.3 (Feb 6, 2025)

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    Download for Linux 64-bit (tar.gz)
    File size: 829 MB
    md5sum: 2cc7128cd538108d2a8a737ec54df484
    curl -o spaceranger-3.1.3.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.3.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=aRm5C8rusWLRjtBXs~1CgLx0kz313DYOyiQaTb739N~dQgsAvF148LGB0owoXRay8~oJ21JQlUJNcc0owf6Q718NWmoDyqcO2kg138ZsPscTZBfce6LdLfhGkffAxIl4yansdyG-DE3rbiqFKIg2iYl2UiSlbXhO-4pSLPjuyBBCbP-A4DP~z8FptoiV9gV4scn5lhetl2jZqIwGNM5fUe6f-c70refZQZGq4mTIZCjNrgHxAExZDgjBPxDs6fmIU8w3GUWlfIFXaAEKoxMLmQjqiOfGzm246kE7kGD7eRrvW1OFpnGSmX4E5tOS4mIKD9nQ7nTMqIg27lCLJVBHmQ__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 529 MB
    md5sum: 2756aa7e3b66139059667117546c41bb
    curl -o spaceranger-3.1.3.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.3.tar.xz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=c34J39335GdyKaQIRGMn4ojZbEI-xzp0splX3~JdzhMTgNxzCLOTO-h0yeoEaA~OuJgH9VRqWPmib85iNiTc2sgPCV0Ai5GuBEsOtYvOWm~NVn6Yj2RoOYUuW7ZBMCZ7mxJFU37qWeJ5XBFbn09GHJMjFIKyqlWcJaPYHuzrPHS0YYqdUei3DQ4CMbTpoc7rRlE-hOBP3pSh1SUA06-7YcVOzqlitbhZJmmyzobOPJDWfhmPQITXUqoEqH2PIuvKQnw4XGFfaosNqwQEA1Pfv~ayo3XOge6d5UnHgfVYBmFzhseanOHgUhs3FuZ0VM9DGRfC3ELKKfUU8umNV3eDtg__"

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Space Ranger v3.1.3 highlights

  • Restored support for AVX1 machines.
  • Other miscellaneous bug fixes.

Space Ranger 3.1.2 (Nov 18, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 867 MB
    md5sum: 949088b1e2cc5bd03079b60a65b41d39
    curl -o spaceranger-3.1.2.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.2.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=ax47-VG6CjPEWg01LDKwc7mTDmAYBrphjmM1ynvNbSDl0DKdnccnqqwFZFnYTPI8IIcURxxezR5pRb8H7dc0dWTg33C1X3GeISyMFZXKPoyyFmo-qP17l0lQ5jp4Vdyzh5LWW8-lh5zSusf8Fv8BWdeQccRtQjEURxJwegxyt2XUOZ6ARrEuUa0ElTZ446P4YDMwYRVaPit1bkoeFktSEOiHBVHbGXmBkPzUEpAwqecrwAe2io8Bptp51jMeBndC-M9CsEGBl7NRpPUgJ-H37QdDwUPNV8PDuWBa9xwaPHFje0-Fa8WoaxKzjw~hb3UIFxU0zCAWiTHSCAekSIWhHw__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 552 MB
    md5sum: 5a923ae250eea2974fd1cef7b2f9c1e2
    curl -o spaceranger-3.1.2.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.2.tar.xz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=KeqwOGvVDKFTQrNMRuQWR7FIpWGjg5-Xv1TgVsBGAOmNNWmuNVjkAT~cbFy0EcxsEH1DdLkyiclxUS5n8id6O1wG11VzcQgXc48BEVye0wFU-K7jTqUNtCWyT-WUCg050BaknRVxbOQkUoGhpChYKed8VhT8NJZEXQWrcHXyZ7EFGJq15a11yqapIlg~BqKMSZVBUTp5G1nl0lQ9q-KTRIuCbnjC~Ex8cuq7o6aafkX8DinKGKkBeoRPzObjcA71WWaUx7M-POZtMIwuOVpnSsvFpwWkEhUzMcLFW1d-3NnOHe95FnzIHJHQwJ7pp7nopYeDfCVJQAiWCpWAdnaE2g__"

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Space Ranger v3.1.2 highlights

  • In Space Ranger v3.1.2, the pipeline automatically corrects Visium HD CytAssist images generated with instrument firmware v2.1 to account for a bug that affects registration between gene expression data and the microscope image.
  • Adds metrics “Number of UMIs per mm squared of Tissue” and “Number of Reads per mm squared of Tissue” to the web_summary.html file.

Space Ranger 3.1.0 (Aug 28, 2024)

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    Download for Linux 64-bit (tar.gz)
    File size: 827 MB
    md5sum: 13a2c82adfb1f1efb737e2be121000d1
    curl -o spaceranger-3.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.0.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=GC3ki1hGMlHUvRLNCIRP9kcD8VhB34isayVsg3jWoYJnxPswzxSQtpcitXYUk3qtoYhkrN7Hfhaba2q5sJJTCJYAjVjdwAwmBHrwLqIlbvXXcgPKuGLHqvcMTtksiy9zVBRtdNgi6i1FfSnXarLTnzSKBaBSh6GQuq3OfDqmTsTLxSqFoPB0-kxNkWAFbARrXGG0MTW9HaGbFe16Q-PzOMq6HveW4ahUO4hHO6eTrSG9~PKwxPRB5QmIJmrjuXgHxc0wFXZRkyoWDlYDBagIdFDN1CgiOp0zI~vfavQ739P~5Mxmq5fTUdfy72mhaypN-PHaUSk2z73YUloK0jf1Uw__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 527 MB
    md5sum: 28010d3724e9effc488470a961fe39fd
    curl -o spaceranger-3.1.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.0.tar.xz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=mps6-ggOH1Is7ydH9tMvxLSCqXB2RlMDkAy24USFNgiZZfpRzD60YK-whocCo7BVvOKTseQPfVPXYUAc9cP216W7dYtyVxnH-Eeh-bXP0dLciejQaXalk3PfXyIbXGdaEIphHMQLqjLyPQ-L3jMOs4y-JhC77COUlzohQyYvw-oOLdUeaSHFdnMWkwer3Ss8a-JD2zHzKsy1TzgXtSEgOMXkxpJVd1Sn2IINl68WRAGtLTC~w9bzeLMbzexq0UmdvST8RjtRXWsKOLVaCTPJ~zgwBH2D7iSTWsie8nUV~AL1VsEjHqZ5V0kfvuV7GCKIt6zKo1Zcp-Yby4JKZqtkRA__"

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Space Ranger 3.1.0 highlights

  • In Space Ranger v3.1.0, the pipeline has been overhauled to significantly improve the CytAssist to microscope image registration process.

Space Ranger 3.0.1 (May 31, 2024)

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    Download for Linux 64-bit (tar.gz)
    File size: 757 MB
    md5sum: ae65e7ffdddbb966277b7d1776f8c63c
    curl -o spaceranger-3.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.1.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=MMkkyACdXIJSWB9GZajFH-xGAtkeZ45Xz8cY4Q4pkhSgZSBLa31gMUCAxnmvek9ssrbqdY4sv5YSvan3qfpT46SP0X7up66ptUUmMUwPrEBmuthyPOXpfQbnwUsuU8NfBqy-2rOj9SbatCEcb9mZ6YuVZxGqXj4pvyu7s7yHSO1wqkzNZTsyx6j3QQogQIafpku9oOPwNdkq-mTM4zNWARTgVVQ79euuNyk1XErK7BiG89VQgC3BflFLB7s6NIK4XITmM0hvBz4-84d8Y1WPqLjWq4SOJ8mhbjQKdWichHf308APjY5kCe4K-iR-2uEJdQyXJoWfUwrRx~EbjlfJLw__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 481 MB
    md5sum: 451495ee2074ff9adbac49ff8851ea1b
    curl -o spaceranger-3.0.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.1.tar.xz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=b-2e~9iggg-enEQ4V76b7FdUQDBx6ieOdFg7REE3M6SMf27kqjYix0DjuZxa~LQT~1az9dtN88bS9eBnWHU5nmVUv~SK8nbdOUAM9iuR4Jcf9bNukMyDuI3uEJp8k4PjFZOL1tvClO4jp2fM203MgAWPNnqcaJfX0DJIXdaBSZIDprX32OntriRYS1p5AUerEe2fGfDkaGE~FXApmT8jq09E1TSh-EirJthCtlUgDuqVGSsjFVjAHP9t8UPUmCRffsX4uRr3CltFJTBGV50rqFwhKrmkGXcdw~cLkfbZzIN78ucV26MMqyTH75LMAD3k1ved5RSBA3fqWOY6lAdC6g__"

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Space Ranger 3.0.1 highlights

  • Space Ranger v3.0 now supports Visium HD!
  • Space Ranger v3.0.1 includes bug fixes and other improvements.

Space Ranger 3.0.0 (Mar 25, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 795 MB
    md5sum: 19f4443f10cf94aff5768bd8968cb214
    curl -o spaceranger-3.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.0.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=O3yFkgbP1vk01NnzcRXpZKB-h2z7zrRVHkb2Cwga3~YgNOYzyp5qPnPRRHvzE4nPz25mPL-D42L5gKcaYpB7ZWv3hISlEZ5jsFpEdWD~zH85DCMC5NzuhR0dgoWBdoOYegcFpf2f3pr4Rngv3XBiDIR8~MY0nKCCCncn5-FjL3-RTv9YvGIGdzcV9vZqTzSOrtjfubTEo3TGMujuQKz6YgVvbFWD93k2P3qFMrhgl8ndS-bmwCrFzGtsCH4GaOHpTLlfkhWAcib4792gvi2L3sw5rs4rwVBlkBwaW1oE5pmM3EFa-B2~FfHQm3CoJ7d99~w9l221Ge4AJiGE0IyKSQ__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 507 MB
    md5sum: cd02b83fe849672066c40a1734819432
    curl -o spaceranger-3.0.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.0.tar.xz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=AyjyMqEu5AtpHZXYtXoymqjBeJZ3jugfkjMenNXvWaf~otPu5LyPUpO7Mn8g5TgpWdadAmjWlPlgD6iI~u975EJwrx5xq1Q2usAh4wvfyTpnJAWnkkXHywaYtuBQYmZ7RsufM4IlYzSD~9t-VUB00m7uXzifYP2gKudOs9r9DSCtdlHOa9UI06Gu0P1SJ2jULUhVkgg6tEmWIscpfEKBBk85Pq3hnA79Zno5tA-ilXX9bYxYmC-nzHNbO1LssuWuY9tCy50h4hYho3QdRUsM0LuA354bbWY1WFeihaGWVo7dW3nk8HkLeAVy8Q0r1HqeM35Z9hpU5tOhE-dyaUjGDw__"

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Space Ranger 3.0.0 highlights

  • Space Ranger now supports Visium HD!

Space Ranger 2.1.1 (Oct 2, 2023)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 707 MB
    md5sum: 0ef64524af2735bd2a0fc9eef5a605f6
    curl -o spaceranger-2.1.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.1.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=RK02B0sr7AxstDy0pt9A-AAhzaD8IDhmwCKcj2ef56WFvSwaQlj0RVcgRlV~NW6kSVz0ieo0UgjoCFHfi8UfJnY7RAWU-3m1mEMMwL~Pc1qXNNsAoC8teP69NY0D0KDafpBQXVanBbMYRVj3x3iVeLRa03cEbbUZOWCjdZpV8Cb0NCO0a7hEnEy52ixEnqOCYSiXm687BtndnOnK~RND4w3ifIJiB0WOWHT~5Y8~PIs0bSupu6PFzZ2h2aArR5svIgWY-1a6~D70y5wD0gcIsxw2sgA939wZYHVkM3tG5nlq22uAoQ15qrMWVe9D58fe~CGruz~gIlBTKPlb-i0EbA__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 465 MB
    md5sum: e1204bb11b3589bc5d5af244b51bfc18
    curl -o spaceranger-2.1.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.1.tar.xz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=ZxV4uToTRHsPHCYbz2d78pOaLDh4ELPC1wx8XO0i7CbY9aDWYtBPGlPZFfKyTWh5feaPKk2yiA~anB3i3YQBp~e7oJFY-HpO7SKQ04BSxoOx06XvuTFnq4nWPfdWWRYlUNxfC0-9YR03IiIGtHmegeRXAPK19gNApCR4xhzmkIG-kYUM7DOfs50DSEWhwgEAMBfd9o6tGsEaSJKJfobqfjj6UlEzViyeRLxctm9JMCvEAp2kXHWr6xPu0efgKL5E7LR~F6CF5CTd9KebSv3~bj1WmxivmHm6vCSlVdQFdcD3OBfoZSy2e6EUd0zsfCfXKoZH7qn4Up8vXZsVgo1E9Q__"

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Space Ranger 2.1.1 highlights

  • Fixed a bug where 8-bit grayscale images were inadvertently turned into 8-bit RGB color images for tissue registration.

Space Ranger 2.1.0 (May 16, 2023)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 707 MB
    md5sum: ee94c453c44770b2b69d6be46b948e3e
    curl -o spaceranger-2.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.0.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=SACSkmyKQ0IwysXe4Wl5SyPZjw7FwaC14dst6Q~pI0J42ddWX5nBHI91ld~adj8sSk~ex2AQTSOK77eZBDgF2JMzMsSWX9yZp17AuGs8kdLZsHEWGDRfYGOpc7nOfUoTe9N5l7bnTnTKbEq7MweFgTlELGHmW9UwtUGRXNq1iwy1JBQ7aO1YVc6BQLIPc8vKxXgCVg1xCxNjPfN-fGo5s6SLQmKE8IirMDrs~AWUe2ZkMYteb5kOBjG0xMjw~2jprJda815u9Dk5LJYz3TMVPysMJKQC7-lkIZjms36UGq-gOTja2Q0pfgtrYIUNh-~vdWLpPm163CMbdp96O~VA6A__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 465 MB
    md5sum: a0d90129a81c41cbdd728e23a4d25161
    curl -o spaceranger-2.1.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.0.tar.xz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=YRIMzVceXkCL0Wo70qZ1LA3W9qqMCWgcXv1qMPfJS3ujFj3WDcl5EOA8t05kwBBRbK4EvV3yiJ3nuBfEUhP154Mc14JrCR1VMQRoUwsR5419buYYNftf9MhM-Wlva9sGVRM6zDDTUJ-pf~MQ8-Isx0eP0yFn-NzKu6WlaJOcIk6n2ame0TH1tLSBglUNlmIpu9C51w2puJfFk1bMOQw3Wcjv9dSw7XFje1mQBQqgNmLlEeWgFPInRhvrjcdZl-gkYy4IiusbuRPuRE5m26wXzLp-2bpYJwEmWKzmtNJACqPhAgx3bWK0966ctWcSTpaH5iP84qKu-jevzjzwwaytdw__"

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Space Ranger 2.1.0 highlights

  • New feature: Gene + Protein Expression on Visium CytAssist.
  • New feature: Reference-free spot deconvolution.
  • Added support for bigtiff (btf, tf2, and tf8) image file extensions.

Space Ranger 2.0.1 (Dec 7, 2022)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 664 MB
    md5sum: 26151804338ae4d4b44151232bd64b50
    curl -o spaceranger-2.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.0.1.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=DinFWvZC2zgt0iij4v1SxXQZwVWGYO1eTnBQ~NuFaJmkX6J0UFdSbwMid6wX5JY0ur2Ah80VcDoLhLSPzsaDBOt2Ne52wTi9nGVtKrnlzgMNzTgojW5rI4OCiFqsuh0YxwNaJbOWBm5LIBm7kcslfphkih5ZoKHS2QJ81nYmt3mWs4S8-rRu5cDYTa2kNRFsxKEOBYSVYNRfZ56JuEXaxjbMRmhxuk64CzunKr6SmWC8eAO2S0C4u7YONp69Fxbv7pZCwrWtbPXGAbWnT85NGFYrqfTTRmaeUSHogVHQKAEGcu6WwtJHR05yyuJtkYVPcrP~TcloXRphtaKjg80FqQ__"

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Space Ranger 2.0.1 highlights

  • Reclustering workflow now supports generating a UMAP projection in addition to the t-SNE projection.
  • Visium Image Manual Aligner now includes additional support for images with difficult to detect fiducials.
  • A new Spatial Enrichment table which allows users to sort and filter features by their Moran’s I value for Visium Spatial Gene Expression is added.

Space Ranger 2.0.0 (Jul 18, 2022)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 662 MB
    md5sum: aec9ec128c7bc9fbf9fd6d31aa390d4c
    curl -o spaceranger-2.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.0.0.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=SzXiNSgBSohow5pHITbdJuO8ElaCxEzlx9iCeDsfdFrToZpmdOGRWsvxWl4EjxO0LvBKYYzNHtyMv4JX1MGSLqG3PFmT76n2lwTmNOYRHimSZS5TYeZVI9XhVj6OnMD9UBBPh~NmLdU-9zCvKyjCx6bO-p3BVYfjxQcPPvbOEYfX4z3QoQWSw-QUXhNmS9SH907i1VjGf4ArZKLkS9GLglyHJ6BCVZtvkNP8pIyjuQ04FSQV~gClNhF~zqEJ6O7anhLZ7iedK~2E2T7j5LYcr4tAlMNoijUwnqkYz05aArokbcotgsD0PrhDpAzpmW7HSxBm4uAj7GZJc~hjhX8qzA__"

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Space Ranger 2.0.0 highlights

  • Space Ranger now supports CytAssist-enabled Gene Expression analysis and includes image registration between the CytAssist image and the microscope image of the same tissue section.
  • spaceranger count now quantifies fluorescence intensity in the image at every barcoded-spot location.

Space Ranger 1.3.1 (Nov 1, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: c2eb73125d9ee731fa825ea3734be1b4
    curl -o spaceranger-1.3.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.3.1.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=jGGEk038B~rseld7aFNX1IxlRayBmo9MCkhe3-29vRHPMECNt5FcgaIM~6iXUXLjHdOkb8VYMzn4-WiSbRynXCO50U-v3MkBtwZpYm5f4nqc9cQZYqiTeix4KTyayk8hRO7KFbDgQvovnywNUx28qFtksv54~AOSHppLymdE2aC73YnAmb4-MMclTxWo~UXN7DWEtaTG2Mf-Wk8dZbUr8YYcmIkJ7vQlr-jNEiMit9YXhpmXOs0Ybiyi2oUi7bG2B3y5duTcDrd83WHDqPwxFxYtQ~bnUSSTaiIs3TtztFLrsDUIZtcpazTYGeN-XeMH17~Iyq1hr3rNqeN~hrtrMQ__"

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Space Ranger 1.3.1 highlights

  • Adds an alert if image alignment would have produced a different result if the --reorient-images option was specified.
  • Update warning for low fraction of reads confidently mapped to the probe set to be more informative.
  • Unsets additional sysconfig environment variables prior to pipeline execution, which may otherwise interfere with the pipeline conda environment.
  • Fixes an issue with mkfastq when bcl2fastq requires LD_LIBRARY_PATH.

Space Ranger 1.3.0 (Jun 9, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: ede7f98db5adf7785e35ae0508c48f74
    curl -o spaceranger-1.3.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.3.0.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=RwqbxUb0Vk0BlZLcYMPWMFRa5FhzIUGq1fKrQAADnqItCM1qq0KFcIhIC-rYIGDhoOZ-3owcsXXglIyHJiPJ8TJkAUQMR2HE~uVdl64Lx2WupNb5ZZ1syS~jXbFp9SoAuoFJEkUD7aP9R1~~a08J2Lp-BshoVI~uehYo23OupiJi3dZM-UinTCrX2HTBnrGiygj1Xrwun6GXkreT6Gc2wlcfNLWOIHnep-ri9MS84JU8ghjLISxka3QwQgSvhLN-42uTLuiH11kSshg7LxsarQVKbaEhXSzM35famZ6riMR0BpgkHpgAPKj0Zvxl~q~w9zdIh93i37DmKlDm95Dmcw__"

Download is unavailable on mobile devices.

Space Ranger 1.3.0 highlights

  • The recommended operating systems for Space Ranger 1.3.0 are CentOS 7 or Ubuntu 14 Linux variants or newer. CentOS 6 and Ubuntu 12 are still supported but have been deprecated.
  • Gene Expression analysis from formalin fixed paraffin embedded (FFPE) samples is now available in Space Ranger and is invoked by specifying the --probe-set option when running the spaceranger count command. Space Ranger 1.3.0 or later is required to analyze FFPE data.
  • Space Ranger now calculates the Moran's I spatial autocorrelation metric on a per-gene basis.

Space Ranger 1.2.2 (Feb 3, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: 3280a085714ce7c894dc14b76312bcda
    curl -o spaceranger-1.2.2.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.2.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=a3jgtNCQzgN9J9LSZt2cB-7NqqQ~zo5xdPLYAMuuWtMWtvuhAS4s0OAXYfr4BCre4KgAammUq09OhOf6FIdK8MbxnRbBb24hke4mlb3fMDwxvz5TM4ZdCHDI2WnRB8OVPP9amKcDp2h4RdD~sB3pIKgJmtd5-EgkgUuviec00fk8OewzMWGWH9ye2QBc1rJb2GfzfJ4PShGMTunGJlt-5WVsgvT2lZqbxW11wEqdjmRWoxhi1IC9tr~pRl0iLpGOGqyVofLNDve2K3C8lFyN-oyGh-e8-7QB0jNXwOVD7Ap7DOtsvWqNa49P0fenMEZCfkfbXQOVQgYaYXGe-aHEhg__"

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Space Ranger 1.2.2 highlights

  • Adds support for pyramid TIFFs, or TIFFs with multiple pages of the same image at different resolutions.
  • Fixes an issue where targeted samples run against new references would fail preflight.

Space Ranger 1.2.1 (Dec 10, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: c24ecfc95c5e4f1c268354c5934da98e
    curl -o spaceranger-1.2.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.1.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=PvB1UXJNCTQ55xjVSoH435IyCzfWqc1udoIzm9ExUH7Kd32A7yKG3S3f2bOK4i0QwaQDmoZ8JePB4PfW2YmBRdmWxKvIYasNQz7JizVEVggSIxpxcZP9pfVffRn2-gRIzD3uxQGOL3L4BAGzPt~G5jUCrVfrk12jXfP4c6uP-ImZe860YpsAEUHqNPWWKlLDvn0IF6eF6Q94D3~Q6A-ovySFlox4jVv-t1dxOpZQe83m9ZbgwgyqwnckP2-Nh7jSkCUhUSNGXzUccMTN6zyqA8YSF-3722dtviBgjJ4NkvfUTPWkQJSSQcwMA3xNMOCJtLZxFWl934xe-HEcK3VvDA__"

Download is unavailable on mobile devices.

Space Ranger 1.2.1 highlights

  • Fixed an issue in aggr where files would fail to be copied on NFSv4 File Systems.
  • Disables multithreading in mkref to address an issue where mkref would fail on hardware without AVX support.
  • Better error handling if the pipeline fails due to a missing .env.json file due to an incomplete copy of the application folder.

Space Ranger 1.2.0 (Oct 27, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: fc21902700779111afc3c813bbb0ff5e
    curl -o spaceranger-1.2.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.0.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=LIYpiur23NrJ6i-qOl9zrw5mm2hyUt9afQu0ko3ZoY3Q9u1-dLQG8i3Usn2qBcHF2ACIcW5x68Kc7yxWqNDeps~3QJ5yT~AAh5bme6f2E8pZInL~hhgDiEYDA6VOu4wcdECDWiGMzvfXgm25TxhUJV8SpumLf48uyU22pqqFSU0xPs-O8qnmlRN51jV~l~O3vt8lD9Qo8QtlHBd0XufQbAIZeQyIKElsNrLxfIOMjeTKXoBVBpr7U3Q-v0PhKd0vfYQtIBKzEoX5V~m~zyz0eq~wOZHDK~gM7flyEdMuEj9ojdT4A72s4W4iuywnfS62gDhfREHuQO4jpL~R0V2lUg__"

Download is unavailable on mobile devices.

Space Ranger 1.2.0 highlights

  • Targeted Gene Expression analysis is available in Space Ranger 1.2.
  • Introduces the new spaceranger targeted-compare pipeline for direct comparative analysis of matched parent Whole Transcriptome Amplification (WTA) and Targeted Gene Expression datasets.
  • Includes the spaceranger targeted-depth subcommand to estimate sequencing depths appropriate for Spatial Targeted Gene Expression experiments.
  • A new command line option, --no-bam, instructs the pipeline not to construct BAM files.

Space Ranger 1.1.0 (Jun 23, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: 708b93997295db31bc457b3d9af79ca2
    curl -o spaceranger-1.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.1.0.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=XwLPB-hhC76QlTi~FB5m0Ycljn5As-iDdH8lKJY1RHuJaqfOZXKoLpijML6xkBln~kUkkdu-HkJ-nTWgJmCtcPovPm1Bnho9mB~xD7tiQhDBh~CxX5ieMdx6gaDIaUuc4i49QMRO5STUQ5d~ULeq3EDNtjYRkBekcDNGVqQbiEBEe8I5sB8FEDh9NVcG5GruSsYgrkLzIK4YK8pB9BCvyCQQWYwBjHpIOUsnAv97ixrMA0CnB-zP3mhDyVl7p57BlEcbIAn86MGHvE~NmknH-aRrJLH4~cLY1JjPuDOGSvgG8sbswo1jllNRc94JiDHdsxgQCrnf4hXVVOP6O4BI2A__"

Download is unavailable on mobile devices.

Space Ranger 1.1.0 highlights

  • Support for fluorescence imaging input such as IHC/IF.
  • Aggregation of multiple datasets.
  • Optional automatic rotation of slide images.

Space Ranger 1.0.0 (Dec 2, 2019)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.3 GB
    md5sum: 5cc7fb86d0cbff7df687b11575efb7cb
    curl -o spaceranger-1.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.0.0.tar.gz?Expires=1765095738&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=NR9-CQINI9Lek3ILDyXweZZA1Ov79oQgKbM9HYXBFf4Km~bmlbdIm6vnrcF8mIpCCt0E1zkIvLcMX1-Ffo1W9LKc4eqskb8rVaNSBQ7OhZB6OgH5Gd~xn--SFW9Sh5TMcXcefoTi9tPEWhOhfYT6s5BHbw2146sIz8hozfTcwP~m0fAnIju5UF9VHYLnJfnHPbe4TAQ4WaJ3F1uwrQs8Jx8vl1z-aXvTVed15-JvC32Hk-nPe4YW3sbIQyhiqcgy2Qxn3J1hZIgGAuxsDnCS-ey4HxzSv1dYP6ps2r-LBVpwZPb7tOXkFlnLZG9NRX1VfwQxCe6-5PQb7EEPZ7MVRQ__"

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Space Ranger 1.0.0 highlights

  • Initial release of Space Ranger.