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Previous Versions

Previous Versions

Space Ranger 4.0.1 (Jun 12, 2025)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 885 MB
    md5sum: b0beb100947717cee8c7ad0d7a9f0247
    curl -o spaceranger-4.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-4.0.1.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=NsiKhhDqhlbzpSRLtg3JvtESdI9PtPksTrFxGQpZvJ5458mrzuvceWaoQImJUwZzOvnJY8C3Q82rGkREU15HsiIe43SbRF1PaxipzOemhPu5wnnlt-PLYgmXOL1KTm154P80yMBugl2qwuEv-13Qw8o1i~AsQOUd4ywsmWktJxddDyXIeemc3OA69Q01rkJ7xrYzv~XdvKS9yTPfVkn6SnXKC4nP-UjBXRBaTAWtJ4JXOlHdZloTVJA13Yc57ESK4IQ0pAU-H5lsuVnxL2WQDXeRHnR-tyd7TRMx6qWQdSXx54SgnE2~eTfbSlXf6iq9AntqkAMxw-PZKYsl5jH~dw__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 582 MB
    md5sum: 3610ff948a6f8129cf49509f1bb34970
    curl -o spaceranger-4.0.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-4.0.1.tar.xz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=eFns2AFVNeSSZdBBx9~rvG6G2I0a77HSuK48WUr-XwefBXDlWbJod4HqYVEe~YsbYDYtxWYK7tem-9S7nPicFQrasFT3-6MzKAnDoNj7gI-LsS1iZT7mVG-IyMGBycGVSLbsQ1lYZAGMUPuCJk3lB3yqsl14wMIbcOWXYr0hM74YcgOD9j8246SBHZhWpPcTfbKQD6VDibbG18xDIKhYtovP903sVehJG41-G5JYVgMpsqEbcXWHcfFYVyDmP46I9HDKAp9-QqbZx~UVhRGSqg3MIq8bDlwKpHjtdNufhaCuV1O6a25R1BupH-3a4sRDzVVMONtLe9knbQt3Y9F1kg__"

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Space Ranger v4.0.1 highlights

  • Space Ranger v4.0 now supports Visium HD 3' Gene Expression!
  • Cell and nucleus segmentation are now included for Visium HD and Visium HD 3' data with H&E images.
  • Space Ranger v4.0 now features a new UMI-based registration algorithm for Visium HD and Visium HD 3' data.
  • Starting with v4.0, anonymized telemetry data is collected and sent to 10x Genomics.
Release notes

Space Ranger 3.1.3 (Feb 6, 2025)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 829 MB
    md5sum: 2cc7128cd538108d2a8a737ec54df484
    curl -o spaceranger-3.1.3.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.3.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=QvlXuCC0PppLvtH3e-67Rfx-SIlmjndEuSFW2~uw-PFNSTpkeIA0xTIHD-U3P2FAdc6ZJDB44mD6m8ZXiCWoyKzeheDEjP4-GJOck7H2KvhCkA7wybNnXBHtmvTWPQ2jyP2m2YRpxmT-I1q~fnGVp-M20cMS3nsR0EFSjCFPp6vpXwOyjTpTSKyu1-tySzmx07ilFspzoLJKjrcygsrbG5O-bgXmXjBSKsU4SNWCVG7PFJ6P0GzZLMngyfndMcUixjSg0GH30BQfRX01IRIhNNTL4oHueVrUdVM5q9DoPEuSx9cbvqF~fJwIYxzQXsuwQZQbSyF8auS0~LcbqqeP2A__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 529 MB
    md5sum: 2756aa7e3b66139059667117546c41bb
    curl -o spaceranger-3.1.3.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.3.tar.xz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=M2pXlcUsLmlNj66aqk666aX0trYfKdvGBkdu2q4zf8XBSlttBBg-nTVYOtQ6QzcOipxSaK1XKT~kRYzw~S6XpBNmmM1rMC2yE46oYHLzA8tWqsU1Y52lI9aZvUGeRFZ75MDeoZrXA4YL~Xbj0HbFsdSosSc4mgCWs8YUDMSLEoDvvkCqMDTP3xg~oUCkqI191Ofr~ZBzkU8dXO6a7dGSeUbeWGQoP7j9V4o7NsTXXSXv2cG4OVj94GNlpCe~4jsHL4I8B9-lKgSb~ZNbQ2wkwL2esiHtoceOB5iB3crE0jjugl8BHU97MlH1-eHnmXeXPcvrF061Zxno1ECkUtDnpg__"

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Space Ranger v3.1.3 highlights

  • Restored support for AVX1 machines.
  • Other miscellaneous bug fixes.

Space Ranger 3.1.2 (Nov 18, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 867 MB
    md5sum: 949088b1e2cc5bd03079b60a65b41d39
    curl -o spaceranger-3.1.2.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.2.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=TLPzYB0zhXMK7-zXlu91AlpwXV-REfXG8T2XVrCgbXzCvfEf-nJ5qAix-onQ5oOz4tIONz9m56~jbqNFMAhKNSAYbNrfVeld1wykmOMzbs2~0WIx-uU14jsHSE5~TfIs8g1ZMyDq3PI20pHDUoU72xSLmNy0lDd~XOhNMWTBuT6~VaZuYshfosHZL9ElVlueAju7urPqqni68p7wgVPUEegfoPyWM6ltjkneElcQuhUGMZ6Ul~e0nsV7LXsv0VVHyprQ5IZHEgkGUFofTzY5SkPZhVrOPd3t5DiZl~tskM69NvvGsYMCNL9TfkI51US1LPWN5V74M8J9u5YV1PdR6Q__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 552 MB
    md5sum: 5a923ae250eea2974fd1cef7b2f9c1e2
    curl -o spaceranger-3.1.2.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.2.tar.xz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=S6w9co2IbRPxy9Vmzuwj1GjlTMqw0dn88beb88u17jPfr15aGBLQLkm0Xqfn6t~iVTGooup6Gs80JOsYi0Sw0IQi8NbWjwzyhCa0Pfo6OVHaDKmTOQdowKcrJ3zyplhgPQcnlujYknHkhUsLqVAbV9Bl2xxMcxK0npHlMTI~5PezfZYu8uDt8rF1z6E-FgNbFDwFEYLVLlfnB-7Y~2B5tWy~31Om8~CVoayH7uapRDvqfMkA8bRDw~Ke-5z7eQPGwgZzigpjX81vPFsnvdG4sFEHd3o~Haexi6L3utLQMaJ6jUGXancb0~SBcdNuE6vMWhmCTuDDsW2XMDI~X~6hAQ__"

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Space Ranger v3.1.2 highlights

  • In Space Ranger v3.1.2, the pipeline automatically corrects Visium HD CytAssist images generated with instrument firmware v2.1 to account for a bug that affects registration between gene expression data and the microscope image.
  • Adds metrics “Number of UMIs per mm squared of Tissue” and “Number of Reads per mm squared of Tissue” to the web_summary.html file.

Space Ranger 3.1.0 (Aug 28, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 827 MB
    md5sum: 13a2c82adfb1f1efb737e2be121000d1
    curl -o spaceranger-3.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.0.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=eHWkQHFmL3oEdAbTm8~B7Zj5yKb39n53BaOJjLiJCyN2~Aq5f73X5B02vZ4D5lQRecQTxeteSy-DOBj-iS6eI2GzakE8wwgZ2DQK7jVSSMx5UAQ~wrnCHA5wGAG54mnLPvREXr7x6-ac9XK4U~guy7IG-v0Q8A8ZUo2hGAo3JvKzzzldl3ki0Ap90RUMuv1aeJ5R~M9chGMwGxnNcQ~JecrBHi3m04nz9uiCc9gaQ~b7WUwUqkyemAn4YO0W6naTiYBgJ8FOa4SP9iEsozE025tuopj3C8HzSOn70xzCJLQQ1fHKGQ88It92rLV5uiqlsiahS2Bicpui8leUif~SSQ__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 527 MB
    md5sum: 28010d3724e9effc488470a961fe39fd
    curl -o spaceranger-3.1.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.0.tar.xz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=ULBRN6WA3sQtjwwIbo1EIEco7wrsNFnmeKx1GQKqnWi5YYyyFxKA4G1JRwXpjRJ3zGmv5wLxIPd3s3ld1FFDenVpRQHPmc1aH9L~NdKsgDdTzSXGuPVquZhC3tEBU3QYGg-4Z1agWCzhn7A8Up4rMWt7OmQDSREbPtrPEhfCXxNNKLQhgelsLsJOv18UUxmFDYeMFffj9nOiSf-xthPDwQ9ruviMBbyxScuNmrlQB4X13KimQou5a2GZhLNgEtfMMpqnn5OVah1GXRdyRgRUBFLWG6GHUfFq5MGI6sKrftTFpOWP6-JfMYyLwZFfQJwBcqYqRYicaLc~x~D5MoQlAA__"

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Space Ranger 3.1.0 highlights

  • In Space Ranger v3.1.0, the pipeline has been overhauled to significantly improve the CytAssist to microscope image registration process.

Space Ranger 3.0.1 (May 31, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 757 MB
    md5sum: ae65e7ffdddbb966277b7d1776f8c63c
    curl -o spaceranger-3.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.1.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=LmnVesbWbzSKaytULgYZpFXLOA1mTBzmPOcPswkbJRos4Zx7PFPMmnu3RSoZZ8vwLXQ65ABgO5xKBjU1~eVoC6SCMM36BvE0SpKfZz7ZgnMazfMcWYDflt8UEfcAbJ1ZEUyduCI3f3HITn-m1zA00LeVB2l4vns12Ycc-QMzAlWkHXOaHetHs4MOpeyXVHP2j8hoJRtrEdrCLg0ZNdEQw8hSSrhv0VH9UlIneXFK2cerSIpYc3~4PPrqYQi912UWc8C9oWYX7HATSO-YOd2r5FW3Ce0KjZVafiyVYS78xv4Nt6iGbBmp~L8H388~VAqmMIg5SIsofZSIt5Y10Cj1Ug__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 481 MB
    md5sum: 451495ee2074ff9adbac49ff8851ea1b
    curl -o spaceranger-3.0.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.1.tar.xz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=KYfBjUmwpeAYgr2RDLEJsJaAze6AjDSxHhkgworOsz8WfYB5ytTlbI8D-3ZxT7ggO97JTqNv2jtVrfZm0OnuhU7XDf-MLFBHfQCh7XagBmCTWzT9mcgAoKXvSW6D7yxRt7-IfXQiF71i13uyvrKecEAJPg3uAt8HaKonECMhxlY4~vSmb1QOmL2PtyXYxfmu6iXjHcRqfdZxsm10Ej0~jXttsYKDt1OHFTiMmoDiSodfi1ONm-BC75ddtFmb92oFqt8C5ShOFC-jCqD3VEwYnhuw~VzqiJe28LWusBA1grcEx9wNfj11Db2ziwKTJW-oogzoPwj4~-3o9qM1G26MbA__"

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Space Ranger 3.0.1 highlights

  • Space Ranger v3.0 now supports Visium HD!
  • Space Ranger v3.0.1 includes bug fixes and other improvements.

Space Ranger 3.0.0 (Mar 25, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 795 MB
    md5sum: 19f4443f10cf94aff5768bd8968cb214
    curl -o spaceranger-3.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.0.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=TC88Gas2L5J-zqgKWZGGVlPoAW-h2bkWwykWDOq21iWSCBCLaEiizZ2kdb00~L09DVeIFipTw12u~QNZus0nVCiBugTrPrHg98xNozpQCsgc6TjZgaS3nmpupTOEZmxs1IwSLCoQV~D2UC-OSTm4K5mUFfW~6AuXr8i4Js4bM1Q8dzhjmDUodfRacpOW9GA5Af0KsYtPbZREjqssxi3Z7MMfQCre4T1m7srqAelZqQFoqzMV~fx8nWEe9Qr83Z8j24vuOO3UyZs~jkuAv-UXEiVUciIhTwVbGcYBz0GBDp1~dnUgRIxpJRqALTXrizw4XKlTgnVocQ1OdWLzim1ZNw__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 507 MB
    md5sum: cd02b83fe849672066c40a1734819432
    curl -o spaceranger-3.0.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.0.tar.xz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=YCvA9P2TA~XEtlNA20~rWlYxXMKeHuRrMWvsAp1-IlKSrnYAbYUgKOOm1RD3YETgcMb4FPDDmmxgNNT3Z-IKQjgFB64OzAJ1qPbR7iglQEpVUt5k7hkJubmIYTosmH4EBEHImLMVpl6EuvHON3y8qFzzw5m3PreMDfYV94vLE2oG7iKi2iFa0oFQzbk6Ehg5Wgduk8OwR637xAH6YJ7R83kXNEofWXUNg03UHOtTxN2GCWD61r4enNmiSSl6SW2jufwcPpfJo54yBOZ4Yt0Ky6ExPdfsuZsHwoVxv81M~h8j4E1yRgYbDndSMxpLvohH9Hc70bkOEdS7-IatrD72rg__"

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Space Ranger 3.0.0 highlights

  • Space Ranger now supports Visium HD!

Space Ranger 2.1.1 (Oct 2, 2023)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 707 MB
    md5sum: 0ef64524af2735bd2a0fc9eef5a605f6
    curl -o spaceranger-2.1.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.1.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=f7PTl-I2cDrHe-YJGp2nMXqsNqwQ5FIs3AVeWgbf0DlyQeYKo7Wr4Y52b0N7FUFYcNyZO6z1xodKTMUL~EXJN9eYfYPwASHFbmSEYmHB0HzOoMlx7fn4Ud7NJZxsCWyhw7IgqSU2yVk45nOUES2FgpgkBmWySiD~J~Jp~CAEdqMhLGsPxtrIVXId0sL7JgMcyIyHeL92yUiAoxzpK7ny94L1gdynoKmxCe-M24gFVJqrwx2lUM04Y2TqGqu9qxAxw1LhPl2UbDID1Ir4J-WhA-u2WjR1cYrE1TQ~-7FMcaoW7I9gC5RzBt2EyGaL0j3zmbwU6eVYpxFqjoV0dDME1Q__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 465 MB
    md5sum: e1204bb11b3589bc5d5af244b51bfc18
    curl -o spaceranger-2.1.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.1.tar.xz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Btwg-~kAo9t2Li-ZM7Re6SfFYlyNUPnakbk0~Lk48dwXhYo1RDIifOQSETMlTzNwRDJqYrJl5e4fiFsocenEtw75XmCfNqavbsVS283ZRwuXbyA7hLvZaM2k9Izp6VDl8VapgXJ0pP7EjsGZeomJY0ZlhbwR8QY9FC2s1t6H~yG~PYvslI7nrSdHVE4e8QSKhZZUfQOX7JipIrbAsmDn4bdchRpYWUJIh2Q-ZGBapeO1jLQvp4JyeBbzOQ4Bs3apcnpX1qgzfLIvMQe7zq-RlZeU62GmJygC4mZYsJEXMEft08-KUxuGjV0JlTupc-YOxDrc6ykbd-JfNL7RJDM53A__"

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Space Ranger 2.1.1 highlights

  • Fixed a bug where 8-bit grayscale images were inadvertently turned into 8-bit RGB color images for tissue registration.

Space Ranger 2.1.0 (May 16, 2023)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 707 MB
    md5sum: ee94c453c44770b2b69d6be46b948e3e
    curl -o spaceranger-2.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.0.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=aEGl2ed0uMgNcOPuAJD3khqdY~xvYV2G1wLuHdOKxNTpyr25-aL~rJVdUuN18hzVpBwG8nbevLUoFnojn20OrXeNNN3-GO6cT6MA-Lxn6n4NVTlng0yGyNnUtimP5NThXP62mCR2H5SAfR4kfuuBvcj9XEG28JuM6baQrhlA4dMJPGjGLkHvXThzPegeco20GIpSvmYc34ELEZY-~l1r-wMdwsUZK8gUrM-u~JWfsvAQc9CXVLKGl9cuQOZtx~uI1sHdI9Py7FgIM44hPzsT6tykdnPx3WAiABdrYYWJMiDS1A4cS21lp2vvzwfib7J-oIW497AnjLOHCrF8DExKKg__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 465 MB
    md5sum: a0d90129a81c41cbdd728e23a4d25161
    curl -o spaceranger-2.1.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.0.tar.xz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=EWJ7Aw~ByUG5pxjV-SkRO1Z4QqFTs7VOO4sCh30q2P~uIiIN~3sbHncN3Y2YBor-xIsAYKhhHzETU7fAO0n0eMPU25DS8UCbPZwTYYG34i5MTJLdXGjer6wwVYh7BCvmipmnfOMDhrZGNBAwkCt4oonW0mXyI75PT3TscfkYRn0BcL4Rn2KynEjwWFD6a6LUB-V2iPFav4v5oa6lPXunwLwjcET2q-n4P-EHf~U1tD2DamLCV1eGAj97-UR5wvy2j9zVAH9zhQmV9LV7yYHpqcOnTjxmskHm0EvyQJfYc4zcNHLuojy2YNgxMbe2sChCedOAstSn4BSOe0G8Mvlrng__"

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Space Ranger 2.1.0 highlights

  • New feature: Gene + Protein Expression on Visium CytAssist.
  • New feature: Reference-free spot deconvolution.
  • Added support for bigtiff (btf, tf2, and tf8) image file extensions.

Space Ranger 2.0.1 (Dec 7, 2022)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 664 MB
    md5sum: 26151804338ae4d4b44151232bd64b50
    curl -o spaceranger-2.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.0.1.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=e9jououKcYLq8BMQD4MZqK47kNvpLgD51Jx3kGgl2Y4b~RzW0GF4gDZ9-Of57ip0TDcJ77HVyqdcp5DJsVjOAIYsY3PCdXIfFyha8aPCNPN~CkUmmN7EbnXGidb~8fN7UXQDV2oISisk3RDMYa-Tjvgec~CnWxj8ef1qdNF8vd9MsL5fr-jUnNNIHcdcpiTAB70ur1ewofdOSitCbmMaOKagM0KQvM8RRZY5mb9Aokaiak17MHw4Mr~SQPkpH2CJ7qNFHP9wzHy-o05jPso~DcsRLwz5n-0swH525uYmlKTIykoTgnXAPng7~3lJBgkqjk6dRvb2zqMunCAdu10Dlw__"

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Space Ranger 2.0.1 highlights

  • Reclustering workflow now supports generating a UMAP projection in addition to the t-SNE projection.
  • Visium Image Manual Aligner now includes additional support for images with difficult to detect fiducials.
  • A new Spatial Enrichment table which allows users to sort and filter features by their Moran’s I value for Visium Spatial Gene Expression is added.

Space Ranger 2.0.0 (Jul 18, 2022)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 662 MB
    md5sum: aec9ec128c7bc9fbf9fd6d31aa390d4c
    curl -o spaceranger-2.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.0.0.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=dewZAEiWKazbZrkU3xNAvMOTQ3CoyQnT3h8mtLyWSa3uFCINLO3aUAtiQyU9Tv2rq9IaMb~ThvfY~DDUH9KyjKOEMea~SsbCqFs9UqL-vrpKGaYT8FGe1a2y~JlALIImrqSNZcM5NBRy9QD8-9cmz1TmQmJuCbzIgJ6aiEtgj8Z4zt8pCYDQxfd0AdVpn~Rvn6y-GU-YiSiHNCn34hzg0ubX2YyACgoDi0bhVDRz1kb-NC8aSW3J9onh1E1UXkpidVbwd08Fe1B4nT4R0zf1Bh32VFX6-VKGgM7qRZmF~Ku~YzVt8WgKQlYXbUyvjIKyR78QNN27Vu65TfK~whSJtg__"

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Space Ranger 2.0.0 highlights

  • Space Ranger now supports CytAssist-enabled Gene Expression analysis and includes image registration between the CytAssist image and the microscope image of the same tissue section.
  • spaceranger count now quantifies fluorescence intensity in the image at every barcoded-spot location.

Space Ranger 1.3.1 (Nov 1, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: c2eb73125d9ee731fa825ea3734be1b4
    curl -o spaceranger-1.3.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.3.1.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=ksKNFidCTMwYvbELfB5XA3mr9EpQng~mV-3bp0irof1fgC4OdzjCe5eIS5KrSXjwLFjrvst4OfWrj5G6Ir9cPl4r5CiTgrkrncoZ5GIytpFVGlEY3SMy-2-Um9-FKy~WvP3lp8fxKsjimvG5NA0TBPYSotGExfz3aBNn0jPToSygsP0CRWc5LzUBCncltlfi~kBSApqL2wD8G4jWBgzSM9ooksnBzzn6xrKmP9dBw1hGrLoE35W2yJZfIy9Bq-6AxMIpgzVSf45bg1LRvfKIaNtcyEn6rFXav5724gr5uxMqRz30VO-kwkDND88yXc~6tpsvBHpXFhW3UDhrJ6et1Q__"

Download is unavailable on mobile devices.

Space Ranger 1.3.1 highlights

  • Adds an alert if image alignment would have produced a different result if the --reorient-images option was specified.
  • Update warning for low fraction of reads confidently mapped to the probe set to be more informative.
  • Unsets additional sysconfig environment variables prior to pipeline execution, which may otherwise interfere with the pipeline conda environment.
  • Fixes an issue with mkfastq when bcl2fastq requires LD_LIBRARY_PATH.

Space Ranger 1.3.0 (Jun 9, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: ede7f98db5adf7785e35ae0508c48f74
    curl -o spaceranger-1.3.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.3.0.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=NaxYLD7r97-EOiHWgEoXIU4WFxSOm0MVfWMgtrM6SdtSVTzMjpBf8mHQLOPz-m5leR-zVFE~SSoKwu~WMC-qHeE-WhNTPpVyL-Nf6VuMKxrqfqFSjrzEUyERxfWcT2BS06RNu78FVfDXCyxgsB85iPV84OVcSIMjt14h1GnPE5no2ugVF3AhP2wvweeuBmR6RwNwEBDzSSegAoajuKYVkK6HIZvP~2fjDhHy3htcjLzAUq7XU878kuGQ2OoSCq8UvyDzHEXrbQx4c0Ab5SfH~oGbkCyepS1V0uyuTFiGWg61mxh6aSVYXvMAECSh9z4iVmpQaBzAQ0q0o-Vylxm4Wg__"

Download is unavailable on mobile devices.

Space Ranger 1.3.0 highlights

  • The recommended operating systems for Space Ranger 1.3.0 are CentOS 7 or Ubuntu 14 Linux variants or newer. CentOS 6 and Ubuntu 12 are still supported but have been deprecated.
  • Gene Expression analysis from formalin fixed paraffin embedded (FFPE) samples is now available in Space Ranger and is invoked by specifying the --probe-set option when running the spaceranger count command. Space Ranger 1.3.0 or later is required to analyze FFPE data.
  • Space Ranger now calculates the Moran's I spatial autocorrelation metric on a per-gene basis.

Space Ranger 1.2.2 (Feb 3, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: 3280a085714ce7c894dc14b76312bcda
    curl -o spaceranger-1.2.2.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.2.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=CsbdIS3FvXcMmijjvf3-4hr7lk9ebbF0IuxStTg1Xvk8EB84Y0uw4qz2Nz1Tq~05-tiMWU2FIgvP3v9W5MWbpvr5rfkVmqRvKrV5fRXvwQ1PnYFbU8ClSOro6Tuvn1aJsnRu7PkLWWObICJGLG8h87Nyu9KrLzBahvWzOHFRtcKvUsI0eLfpYwJY~GAL4DxNusHzixA9H1q1RB25UKqX5QsqoxQrVIZeLhD3YjMU6AQPpW4xvLUnd5EbQhyh0arD1UkYzdBkHDhPDtPufQDCSUPh6ssZ~ITq~QDu8HHTRmrn0LSBJTsuU~5AQvddDpf~TYPSiFTJu3PwMQvDZ8DqEg__"

Download is unavailable on mobile devices.

Space Ranger 1.2.2 highlights

  • Adds support for pyramid TIFFs, or TIFFs with multiple pages of the same image at different resolutions.
  • Fixes an issue where targeted samples run against new references would fail preflight.

Space Ranger 1.2.1 (Dec 10, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: c24ecfc95c5e4f1c268354c5934da98e
    curl -o spaceranger-1.2.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.1.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Rp1Tj-zoD9AXJ4StshGrcB~PKOHrjn3IL9HQ664m9unP7PX2Jrm5VMib8TIJnn~QLLO4rRDVIEiy3RYQM6P0PdS-KP3koOA3SEvCaGC-jP5v5ykycYkbxmsXhU6HYiJoKGWB7G5usHJWqq9dY5EyvmIRf4MXqTil2hNvERMwkSiXets3N6QGyFEGELN27tSTtEI-cRYoFmG2d3OyYHnsI~WAnS7rAzHkiuEFAQpHq43oOLA9r5U-1pEaU3SSTxqBaQ5FH8INf70hkvs3dAfwyEpwqm9VdykW74e0tjN7tAu7jm1wA~cAoje4DzdW3Dr04-E3viq5JknYDerwLQ7t2g__"

Download is unavailable on mobile devices.

Space Ranger 1.2.1 highlights

  • Fixed an issue in aggr where files would fail to be copied on NFSv4 File Systems.
  • Disables multithreading in mkref to address an issue where mkref would fail on hardware without AVX support.
  • Better error handling if the pipeline fails due to a missing .env.json file due to an incomplete copy of the application folder.

Space Ranger 1.2.0 (Oct 27, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: fc21902700779111afc3c813bbb0ff5e
    curl -o spaceranger-1.2.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.0.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=niMvdDjuCtP-pD0ThmbKSJwc8unKYgVqwmRCaITRc678J-ysegRq~WU8rAoA0iTvczbcPXoRxDCs6ZhAARCRuoY46WlqxCbw1LnfUT8pxleZY3rgT8qqHTsz~niB6IOIQYEG-ttfv-whCentvY96I8NLHgRWJVZttDH8jsneOcW161juw7ov9po8CrOWWs8JQVHEgtvMpnM-5H7KkZAKGe-RXVFYVrlls2XXuyewZGvj-AL0hBRna7Y8n0bJx2D8TGn~Ura-GCLxrgjQSuCRtLiJKcUHm3~NM9HBVQPdabZwMkbNlJvFALJ93XIeCE9wyTVN8dQuS-9elx-pLRSBHQ__"

Download is unavailable on mobile devices.

Space Ranger 1.2.0 highlights

  • Targeted Gene Expression analysis is available in Space Ranger 1.2.
  • Introduces the new spaceranger targeted-compare pipeline for direct comparative analysis of matched parent Whole Transcriptome Amplification (WTA) and Targeted Gene Expression datasets.
  • Includes the spaceranger targeted-depth subcommand to estimate sequencing depths appropriate for Spatial Targeted Gene Expression experiments.
  • A new command line option, --no-bam, instructs the pipeline not to construct BAM files.

Space Ranger 1.1.0 (Jun 23, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: 708b93997295db31bc457b3d9af79ca2
    curl -o spaceranger-1.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.1.0.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=MykFYCDiW-NxU0yDEO8qTpH9cMPYH63dDSULHbET~M7h7yO2pghQttCH~U9DLNLTSfkCawq2rDFl9En3Zvzw3dn0TZYudWUnHhOM0aPLkaxBr4PVVRTD23WLa2rGFnphTcCC8ugMDsdefzeTv0t9ohsugo0IUPMGddFRZgAQ0huIaHfcpGQOj0~JkYg6lDbzzFKaljnZ22dT7n-rHQRkWRJ69hSYzyazw7qSolGouqgUy5QCAZz1tEYO~U3wFvfS4BPSeyYUdEhYFOOxcQ96TRr4GtaHEOB30i~gp88qlxuhr~6lM4nswcSD1RsmlCwNJbkz1huTbpIaRV~gj3XWDg__"

Download is unavailable on mobile devices.

Space Ranger 1.1.0 highlights

  • Support for fluorescence imaging input such as IHC/IF.
  • Aggregation of multiple datasets.
  • Optional automatic rotation of slide images.

Space Ranger 1.0.0 (Dec 2, 2019)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.3 GB
    md5sum: 5cc7fb86d0cbff7df687b11575efb7cb
    curl -o spaceranger-1.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.0.0.tar.gz?Expires=1765092788&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=W1yJxZA8VkEYm1VpklbSLIXpYT7vTwJgwIrkAypPlwf3Tq83KUWoeyXcYs7wEIvPryAAiaM3AiwuciaQhFSmBIdLDMJiHN28hBBMGFZNZcNYrZYME4Z5JKdKDh~PMABACNIjiVgCrk7W63Hu2-k3ag0YcDtl6CvKHdq7rvl2fQNR8AY9Jxgi1dGu2HeSq76qN20Wgi0fCvWDwlNqVnXlVL0P4mm-unq4QYWYN0gy3P6P1nHPK9orKXgJFXNz-MyFOqfoUDieEzGNcj7dm8z6EDW1q-ZqQM6ELlZaTeToBfyyD87e0fQgIO~o-x-5JkKqgqKlhXg5A4v4EP6rPfs1~A__"

Download is unavailable on mobile devices.

Space Ranger 1.0.0 highlights

  • Initial release of Space Ranger.