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Previous Versions

Previous Versions

Space Ranger 4.0.1 (Jun 12, 2025)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 885 MB
    md5sum: b0beb100947717cee8c7ad0d7a9f0247
    curl -o spaceranger-4.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-4.0.1.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=ebsWHj-wxM6fhFENOtCWeT1LS8re318xsBi4ZK3u84yA5~DwjeSoPTgGQ83n0vvwVEKog2snJRP~n2xsmIEUC8IpJttYQQdqKZP1AtLiFWQ~e7A4H07Zn73mKHa4OE~~DKprg-Gs0ag~qDdjUVnURF~8UOZ2DB6qwTM6H9Q~mDADp7GMkHfE9ujHrOmsn~DgW6IZ0HAyQpSqNSALmoMJbqGcQXrhs5UOF7-ubgc1iaWgRli~~9au3zznm3omEIzBgVCmKfCOepM9yWo3sQVpRZvdK3aZIkS~97CmwRAS4p5XRqZgUhOhG2RJ63dATnJ4bs00Eo91bETWjIyLMDZT8w__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 582 MB
    md5sum: 3610ff948a6f8129cf49509f1bb34970
    curl -o spaceranger-4.0.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-4.0.1.tar.xz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=kslwlkG-I8wMwaKF2tnaoVDTULpXmMuLNe3ugPR6x3UKwXWDkZAPcKJPLwPAiLNakHoaULk8Bs3a1VM-fR839ZiEXXOuZB5Y~trc03oHtYQFrHNlGgq~qfgECPccGhHhzUQO~-FLXOzEn1jw9KXX-DJOfOB0s65FmNtvXd1mbjm5Ebke8vHYJmow6-COGAOuoq2n9QpeELuNjnxKH7MWll95RHSm2Fv86mou4JIcVBF58iI1Y83DoinpAJY4VZ~Q-NFaSfQIlhKMCNDbq2d-pL~RPQPebJ3n8W2hprVP~83fuyLzHe6DQ5j1SBXKTPo42SgTF3VGxKfr5FBj6fFyjg__"

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Space Ranger v4.0.1 highlights

  • Space Ranger v4.0 now supports Visium HD 3' Gene Expression!
  • Cell and nucleus segmentation are now included for Visium HD and Visium HD 3' data with H&E images.
  • Space Ranger v4.0 now features a new UMI-based registration algorithm for Visium HD and Visium HD 3' data.
  • Starting with v4.0, anonymized telemetry data is collected and sent to 10x Genomics.
Release notes

Space Ranger 3.1.3 (Feb 6, 2025)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 829 MB
    md5sum: 2cc7128cd538108d2a8a737ec54df484
    curl -o spaceranger-3.1.3.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.3.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Hq6aZonOLaiVaHl66Tf6WnCoZ0c4HyaKm6QcjdpnmLSrJhzfoOm7Bd4LN6LPi7gNQP2yaK-dgbz0PR2JOISb1lAGT-5nDruAJzxCS-dUMuPVTxP5P89wax-IUhDsJlp7lSiZXxhCp5z2PVmT4YSnJOZ5QXoiwX7eWs6XICnzR~E42sSsqk3hkM75ib40D5y5nnlU0GgtaTlduQN9qNacs3mcSyjhw8OWJYDA3u5bb6hRdKYUZC4LdfWSQUeqE5I0a1Kfi1msSNYEWCeUp0RiJuzIIqkXaVVdZO0FITW8qfaZY3DQuYz~i1MNJevQ0c7ooZKoHDSDc6krmu-gRgYVDw__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 529 MB
    md5sum: 2756aa7e3b66139059667117546c41bb
    curl -o spaceranger-3.1.3.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.3.tar.xz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=K6rWaW48dN1OaV~~N8oThWMRmuhDHKRbWhurbc5YVRa4Qczwp9AOF3-LXNL7DUy9D31Pz5KtOFpWb4rWCulUXKiiG9dSxxs4Mm23lfqyG5bGRKVvXwD~jMgyNSRz8XiM-DOTFMAda8~obXAXHXG45W0Z8fxrFpl0xiONR27tJgEaDYw5ebFCLSPBmSuMGTByhGB~Mg6wWR9Ehe~8CrfB1D4x23P9XW62iWtp4xqindAvMAmYbOBXoBRDBtrNzk1Ek7DEHrNI4unHZzRlBHLywmudHLMveG4kPkyz1wqb09SVtOMcvIUdYkmLRe~F1sop6I8cf-yPXcP6lomjQIgSrg__"

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Space Ranger v3.1.3 highlights

  • Restored support for AVX1 machines.
  • Other miscellaneous bug fixes.

Space Ranger 3.1.2 (Nov 18, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 867 MB
    md5sum: 949088b1e2cc5bd03079b60a65b41d39
    curl -o spaceranger-3.1.2.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.2.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=L~~whz0dverWNw~DOoTf08oAKBW~AJfuL1ANrJy51uux6Qp9ClhHIciPb8RAKto3uC7c89yghQNblhgxiVEn-u6Be0W7om3I4Ia4z0Bs~6eKnKhtTk9A-QRvYoi4jIglhBfTx1Zd17hnXgCpGcJP-nRH3m1ev56mb0djI8EFa332LfoAZZU3keq6ghPcHTC-Hy6rStHWti5SBAaJf9jdXC32CioW8lLfPLpPXuLBkp-fk1HCZvXObCR7yq0txmDVPZM2bVkoSZPoykGtRwGbYwdT32OYwEauot15Lxp~AOjcwr5FdOi9dj5AKdkwVjDoaRnt03OtLk4TB9a11Q8nTA__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 552 MB
    md5sum: 5a923ae250eea2974fd1cef7b2f9c1e2
    curl -o spaceranger-3.1.2.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.2.tar.xz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=TuGi1svLOY0l5k7TJW1lvpJVWH~wC-HM3ZUGX3dtrTm5ILe890uzcp12mXs-0QT7vI69Q6ti0aGkl99rAge2e7ehB3h8Ds3DHUd93oSqp~vHsRU4bnc8U79pmp7j0UW7fweWoq99-RULWT-Ft~Stkv0fPJT6y2CHwW-VnJOwopnscC1KuzkUjUhyKN~d4-x08pWyNd4objPnDGEQMKMT1vV6rjR4ZX9gTh6CvCbEUegsAR1lsevGJKgTsfke7vOOlL5jkNMcXbpUpWdQufBvUtRCXeivXB12CbGfBP2gYCKbpSB1hQCGBlnPMGL-SeXqP~Xvm7chqy6jqggqeKXfQA__"

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Space Ranger v3.1.2 highlights

  • In Space Ranger v3.1.2, the pipeline automatically corrects Visium HD CytAssist images generated with instrument firmware v2.1 to account for a bug that affects registration between gene expression data and the microscope image.
  • Adds metrics “Number of UMIs per mm squared of Tissue” and “Number of Reads per mm squared of Tissue” to the web_summary.html file.

Space Ranger 3.1.0 (Aug 28, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 827 MB
    md5sum: 13a2c82adfb1f1efb737e2be121000d1
    curl -o spaceranger-3.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.0.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=X7egtTzQgrAR0IMVrGRJJK6nh2Ruc1suQL4KJaQhfYPNbYJpHDfkHrjzahN6jisfBmX0XbELNg58jSHD2es4wrBQ8AnnJDzbdHG~M8sPmcO6QBSPgp9EvN12t8AWnZ8teEkMElEDvXXDOb4spDOz0GXa2hn~riZQXwaN-3ctINa2d~PgPV9YfAiy4AoBoS-RsQI7wkRu7KELe7r7PFfhuX01fGa27VHD~-JbBUZTfRlWPQnR4dIJ8aVNshBs7ay6NsFdJe-mYQF2AptYM99OXlJQibolerOwghtKRZlUwwcYbs~1UlsF~KEj92k6-bn8eGrWfGH2Pl3Y8ooUR5czDw__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 527 MB
    md5sum: 28010d3724e9effc488470a961fe39fd
    curl -o spaceranger-3.1.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.0.tar.xz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=MWhyyAXpC1FnCOQX2j3HKMiOy~LYnZoGjvMJM4dnfvHIzDP-LmgFxBJdqCSBVZnV2a~Xm~N5zZYZB8Xs1Md8PgpSLvuuzfgytNw1AZw8ypjZ3GkjNF4povBJ9XT5DD2ioU4wv2UMhm4Z0RnPnYQYxUPqfNq~vM~2V~PKAaJzfCOwmpnFXGADIk~hDjuefeXIWjh3DM6V8jAZomSMD7LthgAxpxfGiVSBJUEQj1DCvWaxTFiqh16ghmBS3NkuaAx7HYkF0TshOFAX1zT~uRjAZdlI2Kg~aEWobYPSa10eSbvoyKFyyyGge-9miu13~xFdI74GrAJ5kHECNY26hHcOAA__"

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Space Ranger 3.1.0 highlights

  • In Space Ranger v3.1.0, the pipeline has been overhauled to significantly improve the CytAssist to microscope image registration process.

Space Ranger 3.0.1 (May 31, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 757 MB
    md5sum: ae65e7ffdddbb966277b7d1776f8c63c
    curl -o spaceranger-3.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.1.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=lGibIYLzZ1ExZiJU3mS9utOtOdXrnbKwS11c7T4TSquvrVwFMMmtyx2Mo9ImoNyvgGijeA9sKT5gbSdtuTNe5kn3aYdIYWYEseRTOzX1dUHC0EgDzPzAzphrFLL4vPoYDLdXKX9B9GJhnaE25ihCndvi4vqv5r6Ttc0fMNBA~p1NgmhXVTsfiFhUtk-VO3zil8bC28Aa7NUxXWmm-rgNrcBz8aA7um~yxzT8C1zeLg9wtn-iof7dRMIaObuKi9oIYh71-TjPvHbN-sg53rUASAaK-3jU7btFHESqshBUinit5zQSbB0Qn70z6ztPOo3oYslyMU2x5zQRgK~sEGFF0Q__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 481 MB
    md5sum: 451495ee2074ff9adbac49ff8851ea1b
    curl -o spaceranger-3.0.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.1.tar.xz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=HX0jXfyMKiJxSSp7spp~fvdAM1i79C5KgWu89ml1zRzisL-694Kq2zLbFfpEqsWtbwWunePvCIoYI8BlhOWMdoVryeFhwCh8mXE-AnVczlxDloH4z1TT9DgZ6DlZ0mGciSTGzlUNdy7Pkv-SNyDM0OuuRhTtCfcXRQ~MU4DIKOHFQHj5~ImV00ZBedRJNrM02HrvVa~nIV7TgvdQWGjBlBY2PyraruMQgjJBtRVnEygBInZTd7JUzesP5GNwEFJDytOy-dG7DypnRQnxC8FS~0wRPd~HURuy7MovsYPE3DenhCUQSiKd8dJvqihx8g27UD0jFeKmC4ju28C2bm6-rQ__"

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Space Ranger 3.0.1 highlights

  • Space Ranger v3.0 now supports Visium HD!
  • Space Ranger v3.0.1 includes bug fixes and other improvements.

Space Ranger 3.0.0 (Mar 25, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 795 MB
    md5sum: 19f4443f10cf94aff5768bd8968cb214
    curl -o spaceranger-3.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.0.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=fvz7TUlJDGkrCCiBfyDOS54am51EFaT3QeeoditLrBjErf5TYX84k238rfG3n1NiIQnvTI6~lMctMxGNidMErLDqpEgMEJPHxq-qw~A6HGM~pJq2PPeuNjhRFbj0DnufdIuKLTPfiLunfcuemEJ2LIlK-7Ya6~jFH6DYmxxha7NBf5ldeEDU7fnpk6JOLZ94OPyuGgsMmwjMaX2A39l5d7UZkWfC~zkftovY6KTGxB1G~IjW7ysexgdrtjCfCIuzv~hHOMc-0sK1PTFlzs22z1QKJonYPmh8qoX5JMwfL-R3mOYjYilXZtP4hMiv9y45ymRgczHaXICGlNEsNY5jUA__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 507 MB
    md5sum: cd02b83fe849672066c40a1734819432
    curl -o spaceranger-3.0.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.0.tar.xz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=N07ocjfa3SrKdMWcVSnj0nv7L3KEKtY8ydSRQEEoI2fdYUYzZtQLarL-OO9VQTxI3mk4dqsL6tXK9GgEuxns9ZVjRA8R80DXgaw-xqqMtFiyOczyMU8RR~~0ZauQ17bUgQkLJBcRnmFzrq9zMlFUeQuQQp~a~Ohl8FtLLAq7XZF-c6fd3tBmWGRJmlD5EoE6zGT5OKkgiSG3-BQLXgN1Z99QmKbekEW8OPI9D2OpPnfiboSGa5e3TvP7PP6ZnStqSGHU2uLerFIZ-wuDWnF~9JhtMwYghXgUvD~wbUU-rAmWTAEhOXeLIy9nHJ6KsoU2YwGQd2AIp3aFEOvjoj5nGQ__"

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Space Ranger 3.0.0 highlights

  • Space Ranger now supports Visium HD!

Space Ranger 2.1.1 (Oct 2, 2023)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 707 MB
    md5sum: 0ef64524af2735bd2a0fc9eef5a605f6
    curl -o spaceranger-2.1.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.1.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=mRt6lAvkBvuS4l7CILmjxE~CSN1dBxocMqU2xKaVupKd49tsrMJ7TQUupbpJ~fQpUKvK1TKYP~WKzTmnqIsFwOCzoBiRlFa5CykVh0kDbwjl4JaETE21DOe3atgUEHMOeZrjTkAFu9nYSSDmLxzhKKz4O-8OBl8MmE-djzbWwo9TL6A2SrTF~dGojJHKYJQIITA3WyziMOU63QvrRSlvux-jTRgwCxpiWCksz9ExcldJlTXuZqqE3VAJMJ~NlwbvMSydoBmukaCYhkGhmoBbYxSlMbFbV-nr71G~JIPOu4gqGsHM1Epg1Kgt97VZygg0Z0Bs8EK7AcHfUY-PzNCkIw__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 465 MB
    md5sum: e1204bb11b3589bc5d5af244b51bfc18
    curl -o spaceranger-2.1.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.1.tar.xz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=b5hjaaRozfMGao2li58j-~QmfSJk2ZoQnpCIuLTpqNYilB4W55D4-pxfz2NoB7SZwHzFscd-YXyQvF6VAP4FVaIdUu1yeVEg2MmWDdHjzZv2wv6VupmwBNLPfaYtPx9IakrLPpwKER8Tk~tMAHmVr0RgJOPjmOMkkex2tsuU1qhDpfRxXMmBlkGNLvnIg6peQ~bIYY5-tqrvJraYIOaYHZJ~F8Sueme4wJDrunsp4GTiKfQ4uEW1jyLhrLpP7Z3mDjMpGjiOH9mb~kebjNG6ozYhA9tC6dlgA4dUzSR2TdliNcjynMm8rO7YZEF33rutNb4tnmkCmJao2jCBaCEvOQ__"

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Space Ranger 2.1.1 highlights

  • Fixed a bug where 8-bit grayscale images were inadvertently turned into 8-bit RGB color images for tissue registration.

Space Ranger 2.1.0 (May 16, 2023)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 707 MB
    md5sum: ee94c453c44770b2b69d6be46b948e3e
    curl -o spaceranger-2.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.0.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Ucc7jMFt~54fhQdm~CnBhLeW0Bts4iKSz5FcG2b2mYmTRQTUzUe8mzYVfjd6--sI5qHJsKokmPB7cXgvJwx3F4q6RvsjGDeuGYgWAlsQxuyDmDi1lAm8HqbbjsuYdw2SRhh9xGgzhP7j6k88MHlgoJvMLUHP4t5hQKkfm-tlkRcwtks9aPhhOoS82nxh7cb4~RIXRurd~rhW0sZD8V82YYIU7lo0Y0QHt16hfAoiY4eMFFJNbisZnmxTqSl7~2cEa9RmgUlsmqsRtAZQuAbDTKZNhwK4oMYPtz4LtxlA2D69brtNeL7jA15gEQdpgf9iSYXBzz2c~8DRL0kGn7gi~g__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 465 MB
    md5sum: a0d90129a81c41cbdd728e23a4d25161
    curl -o spaceranger-2.1.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.0.tar.xz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=jYVuakor65XXppkPX81Nns8p4CL58Egv6zk~uOqQxJMrB-OpO9LMEme4MAtvntU8SbeQf48WoGkTy2MmgglVpye~-kC04LaiauOyqq6wN~vgPtKehU3FDORsCmwwW26iFB6c~1eAhtOziYJ4GFyHu8k0UfrpjalmD-WFwPw1KBxFJtjcznuWzLh1e9LbiDdAtqhYDiz8TPf621p-gx63wNK7jCzjtdGjyQX9fc2JUcrXgF7DN3Sr6izL4yeYpOABymSCIFmNBkCXzcFFCYcR3DcuzOBkDDOY1Qcm4FinoxAw-oG82IAdJHt6fNA8Rgn4J4Ex9texj2IhT39-ufOtXg__"

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Space Ranger 2.1.0 highlights

  • New feature: Gene + Protein Expression on Visium CytAssist.
  • New feature: Reference-free spot deconvolution.
  • Added support for bigtiff (btf, tf2, and tf8) image file extensions.

Space Ranger 2.0.1 (Dec 7, 2022)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 664 MB
    md5sum: 26151804338ae4d4b44151232bd64b50
    curl -o spaceranger-2.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.0.1.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=YOHXuhLtXr2q01fMUF9oVQZ0oLhhGoSjIDqZS~Xg3FX4c-DLw5rA72O6oh8eqRiAs4ceiBqE8KU4qEZ5XxC7JT1lLXK-0PNSq7hheULHQjBNZjNOCE6r-XtId-DV3rBrc4QqoP~FGB29F3FOyKbTsctAapNQrZi8KzqGMGi37gArM8duZMbfgDP7EeaX4osFCMlXIQlKgnBE~OakO2Gp2o0weG~HY8TN1gECIg38VCY6sMEDOAk8YspOsJ-mvIRQo5MnBKvgrKN6ysLSTelgcaVTLOMR6u5BY5rxNg~UhxPn4KeZmgSLZTZjcEssRJuEuRmNQV~O1GD5b6K~uAJiuw__"

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Space Ranger 2.0.1 highlights

  • Reclustering workflow now supports generating a UMAP projection in addition to the t-SNE projection.
  • Visium Image Manual Aligner now includes additional support for images with difficult to detect fiducials.
  • A new Spatial Enrichment table which allows users to sort and filter features by their Moran’s I value for Visium Spatial Gene Expression is added.

Space Ranger 2.0.0 (Jul 18, 2022)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 662 MB
    md5sum: aec9ec128c7bc9fbf9fd6d31aa390d4c
    curl -o spaceranger-2.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.0.0.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=P1GpMMJuRjgTS8upcaTdCMGQiq1nigYxNcSYSPWfL9xNKUidkXBBNJP6qJXPHVgDcr8h9EHxiWxS3v1mDI4PI7xgjTQmTkWdC9CJ52AQCI8u965tBFl6zpiSXOvInOiTNQspA2IIVyD2f88R7~HppwPZv8ubvM07kGDj6hdk~E8JVQ3vRvXaFj2qigZMT0kEe~uug3qE05g2z-Mp-gr5fR~hnHo4yoXqdnkaTmkHeNGdPUQhC2QJnJ7A~hjZm-b~7fmDSi4aDt7Wo72~R8PMEa6WEhKbOfvxBj07sYzEqf70YvLjlwtH7R7cbmKaLPsIyt3zy2I6HCGA3VzDK36G6Q__"

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Space Ranger 2.0.0 highlights

  • Space Ranger now supports CytAssist-enabled Gene Expression analysis and includes image registration between the CytAssist image and the microscope image of the same tissue section.
  • spaceranger count now quantifies fluorescence intensity in the image at every barcoded-spot location.

Space Ranger 1.3.1 (Nov 1, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: c2eb73125d9ee731fa825ea3734be1b4
    curl -o spaceranger-1.3.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.3.1.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Qr2e5zXYV5f5sW590~SX-L88ek3geVvyN2XC-n-OcoE65kIIYn1jpE6REYUyD92PSKTmQlJd1XTeuLnJsggvKSLUR1jATJS2NJ47sNlz3y-n6TbkE9-4s4xu4SQzZ4jxq0C4Re6yDad5diao4vxNw35WXxmEmZSkW19bvXR1cGV3TTMEANsc~ErD58KTvvPdg5onrtXPExbEraWLs9zq3Y1MNsU1RJqmZTrUc97Q7nnKXGJTibu3QkMAFLOHniKagH4KyBS-TdDmWjLqKl3nZUkkS0NiEvIYVQAK27W~GtsomOQP88fLGZKHA4KX5iBYPUcO7W0VoguoxhG1ai6bWg__"

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Space Ranger 1.3.1 highlights

  • Adds an alert if image alignment would have produced a different result if the --reorient-images option was specified.
  • Update warning for low fraction of reads confidently mapped to the probe set to be more informative.
  • Unsets additional sysconfig environment variables prior to pipeline execution, which may otherwise interfere with the pipeline conda environment.
  • Fixes an issue with mkfastq when bcl2fastq requires LD_LIBRARY_PATH.

Space Ranger 1.3.0 (Jun 9, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: ede7f98db5adf7785e35ae0508c48f74
    curl -o spaceranger-1.3.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.3.0.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=FwCZ~dBaAd2-P1Zuech3sWMEt45SmCLQZu8nK6dG8VpylPoOW7oBx4cpCYOzcB4u7xphaPaT~5u9Qh98ZZzIlLzn2mGPYyaB-lQilCJgsIdjkoGiWiPkXeNi~1XYV0p6IqY4QCcqVnjbVl2xT3Pv1myzwCNVavZxvlZUeq2FhMsCd2vdrFgvq249g5XKA9XKeLaR~zE2354hSTMApa1qFTRp0mk7ZMw50ZIBeOQlVou92CsBYMlzM7Wxejg9B2Jp7he0Dt2KC66~D6ABKluCTipdK6sA3yh6M9QWlPv5bBvThHcrkNSan1BaBDjplKoOvnm6Ot2mJiJM5VWT~gOPqQ__"

Download is unavailable on mobile devices.

Space Ranger 1.3.0 highlights

  • The recommended operating systems for Space Ranger 1.3.0 are CentOS 7 or Ubuntu 14 Linux variants or newer. CentOS 6 and Ubuntu 12 are still supported but have been deprecated.
  • Gene Expression analysis from formalin fixed paraffin embedded (FFPE) samples is now available in Space Ranger and is invoked by specifying the --probe-set option when running the spaceranger count command. Space Ranger 1.3.0 or later is required to analyze FFPE data.
  • Space Ranger now calculates the Moran's I spatial autocorrelation metric on a per-gene basis.

Space Ranger 1.2.2 (Feb 3, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: 3280a085714ce7c894dc14b76312bcda
    curl -o spaceranger-1.2.2.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.2.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Olq9Wm8jII0ZsNZNsg8nz6xnW18o9a8-r4mOq2jj-AMIQqww6~NxJOx3m08jOGukhPZKxE-zT2jO6cWznXQA59K-S-ONqPMQo10ycZcW6qZKKSc9v5SbFehhCzs~cv99wW0dGkesH1JO3aQcjyvV8zu4ERzq5Da0fOrS1vSCCbEs245HxMEDE2yWvABJf6zcCZzQQSEWARLwq9p4yz00PfdEEBd-doWUcSC62AT008XGoUGIM~YwHd4UnMfS4vKutiOun0-zGdWmdQ8pYGDSmkemASbDvRe0FpZ9Mf9gI8J9~LPNbpJ8HtO1deSJScJtpHaD-3WHEL75FfnuT8VDEQ__"

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Space Ranger 1.2.2 highlights

  • Adds support for pyramid TIFFs, or TIFFs with multiple pages of the same image at different resolutions.
  • Fixes an issue where targeted samples run against new references would fail preflight.

Space Ranger 1.2.1 (Dec 10, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: c24ecfc95c5e4f1c268354c5934da98e
    curl -o spaceranger-1.2.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.1.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=IfSo6WaFdOI7TE22~LyRMXewgqvhL3AlbHwNYSqLSAVnt9Y-cotlbAysx50HLPnzP6~uZTj9Gpiy0avJvMLC4MvuRGHLCuDw3O9q-6Yk2bjGTQaB2lxq8ZJMgKxSD7Xhu4l8najIphcNp-U4uALdQF4wqxA3~LmQY5TFJ~nyoE2-Gr2hzovRns~m1ze918z7vlUxxcXSgMsKj9bLoj6apEPqnTeIymn3PmRNdRr9huh5~68vYXPTKcEuxmsaY8KVaaN-Yqh2aSny5dUH5-8WV0-63-lSxSbSPmEdaTxn93EFPX9b3iu4avrf~buPizQNsKoD2-dIi9LopnCCUwi50Q__"

Download is unavailable on mobile devices.

Space Ranger 1.2.1 highlights

  • Fixed an issue in aggr where files would fail to be copied on NFSv4 File Systems.
  • Disables multithreading in mkref to address an issue where mkref would fail on hardware without AVX support.
  • Better error handling if the pipeline fails due to a missing .env.json file due to an incomplete copy of the application folder.

Space Ranger 1.2.0 (Oct 27, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: fc21902700779111afc3c813bbb0ff5e
    curl -o spaceranger-1.2.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.0.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Qn6UvJ8OviKshYyfeUTDf73PT5UwfjVKqKa~hO~-KbOGj213fkWDMZDSWFJMvVp7hPmL6rpmH~NEw4AIxY5EDjz~IXOz7VbZHn04Lx6wI4K1HvTTVcQX4Qw7fG8eSuh3g6S90JhYqY~y4I5m0cXkZAIt23QIanmY7Wg2aF~7pW3LpwuyIw8Hj8hLtvt1xx-mauhj0AqIdIxf-D9AKC1vzcr6cM-J83vSaZwomIzsGt70~md8LfG~XdK5dBBUCLv0jxbot5qCjkoa6--InXwjYBCnDVpnvvixgk7xRZDnBDBEDTeeg4tngaTMNIkLtaLbr01lvHqr4mkWzeqQtc0pxA__"

Download is unavailable on mobile devices.

Space Ranger 1.2.0 highlights

  • Targeted Gene Expression analysis is available in Space Ranger 1.2.
  • Introduces the new spaceranger targeted-compare pipeline for direct comparative analysis of matched parent Whole Transcriptome Amplification (WTA) and Targeted Gene Expression datasets.
  • Includes the spaceranger targeted-depth subcommand to estimate sequencing depths appropriate for Spatial Targeted Gene Expression experiments.
  • A new command line option, --no-bam, instructs the pipeline not to construct BAM files.

Space Ranger 1.1.0 (Jun 23, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: 708b93997295db31bc457b3d9af79ca2
    curl -o spaceranger-1.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.1.0.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=dcEtcGRqAvIapk9tqNHJeVbi59sE00QtJDD6AmEqlfIOvSAbUcfNM~LaWCRR92j5B2Mv3DXNxePzdWeyd82fIOzfrz04EWny2GyfouuXOD3AAK7J4w7n4V-IAjQvk1bbMaoTqFn~bgiUmwoD~zbDPSUefIlSLVnu76ZBLC2sG4YhXEC~zp64PrfMKu95T5tBJamuPaZpHW7p4o7EL~GUNGoRPm0CSOphZkhUJ2ebRKwA9sTkwxkg8Odx6azxb6KTDglhPDJLIhWiXy9i5WYlfYFYi76T~LFh2jSq7e3hOdpmZ6iWFib2csBpbnPLGUlJi9--wRVjI58QHzZutFJZdg__"

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Space Ranger 1.1.0 highlights

  • Support for fluorescence imaging input such as IHC/IF.
  • Aggregation of multiple datasets.
  • Optional automatic rotation of slide images.

Space Ranger 1.0.0 (Dec 2, 2019)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.3 GB
    md5sum: 5cc7fb86d0cbff7df687b11575efb7cb
    curl -o spaceranger-1.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.0.0.tar.gz?Expires=1765092851&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=SmZVo2~Cms9jLFWBXriHwBPFmaofMki2eZrGP3gY8X~QGJNo32vKi8kYndi4E~AavTlmmRN4aIETWml6oeKIrOdeStYgUBmcHSssJOFznG8h4x0NizYLkx9DwrdjWBrKxpkOZ~ebVR7n~kggyHcxDJzfmnSWHAGLdo~250E2TW23Jj0D0WZF4Ivrmkk9Nd2zKjsbcSjafAnNMDb01s~1CokTl1H~cLsv~FumeMxEseelBxC4de-gOB-0~6A4TBRKUpOPUce94BFM0mA0Ky-K7JhguTt7EsAVeXurb6jWWpnuGDsURSQqd6nWIDCAUFnanOzxwJaMc8H3p9Uk7VSQVw__"

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Space Ranger 1.0.0 highlights

  • Initial release of Space Ranger.